CentriPure 96 Gel Filtration Column Array 500 Replacement Columns

CentriPure 96 Gel Filtration Column Array 500 Replacement Columns are supplied as single columns. To be used to exchange columns in CP-0582.

The CentriPure Gel Filtration Column Array is designed for rapid and efficient removal of small molecules such as dyes, salts, biotin, ammonia, haptens, etc. from larger nucleic acids, proteins or nanoparticles, which are simultaneously purified and desalted in a single step.

The solid phase in the CentriPure 96 Gel Filtration Column Array 500 – Z25M is Zetadex-25, a size-exclusion gel that is suitable for approximately 10 base pairs for nucleotides and 5 kDa for globular proteins. It may also be used with spheroidal nanoparticles greater than 2 nm in diameter. Entities larger than the pores are excluded from the gel, remain in the void volume, pass rapidly through the column, and are eluted free of low molecular weight contaminants. The removal efficiency of small molecules typically exceeds 99.99%, with a dilution of around 1.5 to 2-fold, and a nucleotide or protein recovery of greater than 85%.

Zetadex-25 Medium is a cross-linked beaded dextran solid phase with an average particle size of 50 – 150 µm. The particle size range enables purification in gravity-based formats. CentriPure columns can be pre-washed with pure water for desalting or pre-equilibrated with a buffer of choice for a customized buffer exchange. The gravity column provides a significantly faster and far more efficient alternative to dialysis or TFF. Samples are processed in less than 15 minutes.

CentriPure 96 Gel Filtration Column Array 500 – Z25M simultaneously processes 96 samples with sample volumes of 500 µL in a standard ANSI/SLAS microplate footprint ideal for automated systems.