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| CAS-Number: | 246256-50-8 |
|---|---|
| Molecular Weight: | 527,5 |
| Empirical Formula: | C29H25N3O7 |
| Absorption [nm]: | 554 ± 3 |
| Emission [nm]: | 577 ± 3 |
| ε [M-1 cm-1]: | >80 000 |
| Buffer System for Fluorescence Readings: | T&S Buffer, pH 9 |
| Solubility: | Acetonitril, DMF, DMSO |
| Appearance (colour): | violett |
| Appearance (form): | solid (powder) |
| Storage: | - 20 °C. Keep dry! Keep away from light, especially in solution! |
| Trade name: | 5/6-Carboxytetramethylrhodamine, succinimidylester, mixed isomer; 5/6-TAMRA succinimidyl ester, mixed isomer |
| Reactive Moiety: | NHS ester, reacts with 1° amines to form a stable amide linkage |
This single isomer non-activated fluorescein derivatization reagent may be used for covalent labeling of proteins and alkylamino modified nucleic acids.
Fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser, making it the predominant fluorophore for confocal laser scanning microscopy and flow cytometry applications. It has a relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility. Importantly, protein-fluorescein conjugates are not inordinately susceptible to precipitation.
5-FAM
Absorption: 493 nm (e = 72.000 M-1cm-1) at pH >8
Emission: 517 nm at pH >8
13 - Non-flammable solids in non-flammable package
WGK 3
Not dangerous goods
Not dangerous goods
Not dangerous goods
13 - Nicht brandgefährliche Feststoffe in nicht brandgefährlicher Verpackung
WGK 3
Kein Gefahrgut
Kein Gefahrgut
Kein Gefahrgut