| CAS-Number: | 92557-80-7 |
|---|---|
| Molecular Weight: | 473,9 |
| Empirical Formula: | C25H15NO9 |
| Absorption [nm]: | 492 ± 3 |
| Emission [nm]: | 514 ± 3 |
| Quality Control: | ≥ 90 % (by HPLC at 240 nm) |
| ε [M-1 cm-1]: | >81 000 |
| Buffer System for Optical Spectroscopy: | PBS, pH 7.4 |
| Solubility: | Acetonitril, DMF, DMSO |
| Appearance (colour): | Orange |
| Appearance (form): | Solid (powder) |
| Storage: | - 20 °C. Keep dry! Keep away from light, especially in solution! |
| Trade name: | 5-Carboxyfluorescein NHS ester; 5-FAM SE; 5-Carboxyfluorescein succinimidyl ester, single isomer |
| Reactive Moiety: | NHS ester, reacts with amines to form a stable amide linkage |
5-Carboxyfluorescein (5-FAM) is a bright green dye with excitation/emission maxima of 492/517 nm. It offers a high quantum yield, is fully compatible with fluorescein (FITC) filter sets, and closely matches the 488 nm spectral line of the argon-ion laser. Common applications include labeling peptides, proteins, oligonucleotides, and other biomolecules for fluorescence microscopy, flow cytometry and in-vitro assays.
This NHS- (succinimidyl-, SE) activated derivative enables covalent modification of primary and secondary amino groups in peptides, proteins, amino-modified nucleotides, and other amine-containing biomolecules, yielding stable amide bonds. The product is provided as single isomer.
Hazard Classifications
Skin irritation, Category 2 (Skin Irrit. 2)
Eye irritation, Category 2 (Eye Irrit. 2)
Specific target organ toxicity - single exposure, Category 3 (STOT SE 3)
Hazard Statements
| H315 | Causes skin irritation. |
| H319 | Causes serious eye irritation. |
| H335 | May cause respiratory irritation. |
Precautionairy Statements
| P261 | Avoid breathing dust/fume/gas/mist/vapours/spray. |
| P262 | Do not get in eyes, on skin, or on clothing. |
| P280 | Wear protective gloves/protective clothing/eye protection/face protection. |
| P302+P352 | IF ON SKIN: Wash with plenty of water/… |
| P305+P351+P338 | IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. |
Signal Word
Warning
WGK
WGK 3
Storage Class Code
13 - Non-flammable solids in non-flammable package
ADR, RID (road and railroad)
Not dangerous goods
IATA-DGR (transport by air)
Not dangerous goods
IMDG-Code (transport by sea)
Not dangerous goods
Gefahreneinstufungen
Ätz-/Reizwirkung auf die Haut, Kategorie 2 (Skin Irrit. 2)
Schwere Augenschädigung/Augenreizung, Kategorie 2 (Eye Irrit. 2)
Spezifische Zielorgan-Toxizität (einmalige Exposition), Kategorie 3 (STOT SE 3)
Gefahrenhinweise - H-Sätze
| H315 | Verursacht Hautreizungen. |
| H319 | Verursacht schwere Augenreizung. |
| H335 | Kann die Atemwege reizen. |
Sicherheitshinweise - P-Sätze
| P261 | Einatmen von Staub/Rauch/Gas/Nebel/Dampf/Aerosol vermeiden. |
| P262 | Nicht in die Augen, auf die Haut oder auf die Kleidung gelangen lassen. |
| P280 | Schutzhandschuhe/Schutzkleidung/Augenschutz/Gesichtsschutz/Gehörschutz/… tragen. |
| P302+P352 | BEI BERÜHRUNG MIT DER HAUT: Mit viel Wasser/… waschen. |
| P305+P351+P338 | BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Eventuell vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen. |
Signalwort
Achtung
WGK
WGK 3
Lagerklasse
13 - Nicht brandgefährliche Feststoffe in nicht brandgefährlicher Verpackung
ADR, RID (Strasse und Eisenbahn)
Kein Gefahrgut
IATA-DGR (Luftverkehr)
Kein Gefahrgut
IMDG (Seeschiffverkehr)
Kein Gefahrgut