| CAS-Number: | 76823-03-5 |
|---|---|
| Molecular Weight: | 376,32 |
| Empirical Formula: | C21H12O7 |
| Absorption [nm]: | 493 ± 3 |
| Emission [nm]: | 517 ± 3 |
| ε [M-1 cm-1]: | >72 000 |
| Buffer System for Optical Spectroscopy: | PBS, pH 7.4 |
| Solubility: | Methanol, DMF, DMSO |
| Appearance (colour): | Yellow |
| Appearance (form): | Solid (powder) |
| Storage: | RT! Keep dry! Keep away from light, especially in solution! |
| Trade name: | 5-Carboxyfluorescein; 5-FAM; 5-Carboxyfluorescein, single isomer |
| Reactive Moiety: | Free carboxylic acid |
5-Carboxyfluorescein (5-FAM) is a bright green dye with excitation/emission maxima of 492/517 nm. It offers a high quantum yield, is fully compatible with fluorescein (FITC) filter sets, and closely matches the 488 nm spectral line of the argon-ion laser. Common applications include labeling peptides, proteins, oligonucleotides, and other biomolecules for fluorescence microscopy, flow cytometry and in-vitro assays.
This highly purified 5-Carboxyfluorescein might be used for covalent labeling of proteins and alkylamino modified nucleic acids upon activation as, e.g. succinimidyl ester (SE or NHS ester). The product is provided as mixture of isomers.
Hazard Classifications
Eye irritation, Category 2 (Eye Irrit. 2)
Hazard Statements
| H319 | Causes serious eye irritation. |
Precautionairy Statements
| P262 | Do not get in eyes, on skin, or on clothing. |
| P280 | Wear protective gloves/protective clothing/eye protection/face protection. |
| P305+P351+P338 | IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. |
Signal Word
Warning
WGK
WGK 3
Storage Class Code
13 - Non-flammable solids in non-flammable package
ADR, RID (road and railroad)
Not dangerous goods
IATA-DGR (transport by air)
Not dangerous goods
IMDG-Code (transport by sea)
Not dangerous goods
Gefahreneinstufungen
Schwere Augenschädigung/Augenreizung, Kategorie 2 (Eye Irrit. 2)
Gefahrenhinweise - H-Sätze
| H319 | Verursacht schwere Augenreizung. |
Sicherheitshinweise - P-Sätze
| P262 | Nicht in die Augen, auf die Haut oder auf die Kleidung gelangen lassen. |
| P280 | Schutzhandschuhe/Schutzkleidung/Augenschutz/Gesichtsschutz/Gehörschutz/… tragen. |
| P305+P351+P338 | BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Eventuell vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen. |
Signalwort
Achtung
WGK
WGK 3
Lagerklasse
13 - Nicht brandgefährliche Feststoffe in nicht brandgefährlicher Verpackung
ADR, RID (Strasse und Eisenbahn)
Kein Gefahrgut
IATA-DGR (Luftverkehr)
Kein Gefahrgut
IMDG (Seeschiffverkehr)
Kein Gefahrgut