Product number: AF-0102

5/6-Carboxytetramethylrhodamine NHS ester, mixed isomers (5/6-TAMRA-SE)

Amine-reactive rhodamine dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 554 nm, Em: 577 nm).

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CAS-Number:	246256-50-8
Molecular Weight:	527,5
Empirical Formula:	C₂₉H₂₅N₃O₇
Absorption [nm]:	554 ± 3
Emission [nm]:	577 ± 3
ε [M-1 cm-1]:	>80 000
Buffer System for Fluorescence Readings:	T&S Buffer, pH 9
Solubility:	Acetonitril, DMF, DMSO
Appearance (colour):	violett
Appearance (form):	solid (powder)
Storage:	- 20 °C. Keep dry! Keep away from light, especially in solution!
Trade name:	5/6-Carboxytetramethylrhodamine, succinimidylester, mixed isomer; 5/6-TAMRA succinimidyl ester, mixed isomer
Reactive Moiety:	NHS ester, reacts with 1° amines to form a stable amide linkage

This single isomer non-activated fluorescein derivatization reagent may be used for covalent labeling of proteins and alkylamino modified nucleic acids.

Fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser, making it the predominant fluorophore for confocal laser scanning microscopy and flow cytometry applications. It has a relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility. Importantly, protein-fluorescein conjugates are not inordinately susceptible to precipitation.

5-FAM
Absorption: 493 nm (e = 72.000 M-1cm-1) at pH >8
Emission: 517 nm at pH >8