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NHS ester, reacts with 1° amines to form a stable amide linkage
TurboTag 777 Protein Labeling & Purification Kit is designed for the labeling of proteins with molecular weights greater than 25 kD (in particular antibodies), using a reactive succinimidyl-ester of DY-777 from DYOMICS. The conjugates result from the formation of a stable covalent amide linkage.
The protein-dye conjugates have fluorescence-excitation and fluorescence-emission maxima at around 759 nm and 793 nm, respectively. Up to 15 nmol of protein can be labeled using one vial (50 nmol) of the reactive TurboTag 777 dye.
To get a quick start with IgG, use the Optimized Protocol for Labeling 1 mg of IgG or the Optimized Protocol for Labeling 100 µg of IgG (i.e. monoclonal antibodies).
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