Product number: AF-0201

Dicarboxymethylene Blue NHS ester (DCMB-SE)

Amine-reactive methylene blue redox marker for electro-active labeling, designed especially for labeling of biomolecules.

This item is not on stock and has to be re-ordered.

Molecular Weight:	722,11
Empirical Formula:	C₃₀H₃₂N₅O₁₂SCl
Absorption [nm]:	669 ± 3
Emission [nm]:	686 ± 3
ε [M-1 cm-1]:	>53 000
Buffer System for Fluorescence Readings:	TE Buffer, pH 7.6
Solubility:	Acetonitrile
Appearance (colour):	dark blue
Appearance (form):	solid (powder)
Storage:	- 20 °C. Keep dry! Keep away from light, especially in solution!
Trade name:	Dicarboxymethylene blue succinimidyl ester; Dicarboxymethylene blue NHS ester
Reactive Moiety:	NHS ester, reacts with 1° amines to form a stable amide linkage

The amine-reactive methylene blue derivative is used as an electroactive labeling reagent for covalent attachment to proteins and alkylamino modified nucleic acids. Dicarboxymethylene blue contains two activated carboxylic acid functional groups. In addition to its relatively high absorption, DCMB has excellent and validated redox properties and is a good generator for singlet oxygen, making it to a particularly suitable label for electrochemical and photosensitzer applications.

*Absorption and fluorescence emission maxima of dicarboxymethylene blue (DCMB, F-0201) in TE buffer pH 7.6.

Formal Electrode Potential vs. Ag/AgCl (1 M KCl): Ef = -0.216 V

Sorry, an error has occurred.

We will correct this error as quickly as possible.

Please select the tab "Documents" and choose the "Material safety data sheet".

Material safety data sheet (English)

Material safety data sheet (Deutsch)