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Cleavage of the oligonucleotide from its solid support and subsequent removal of all protecting groups from the nucleobases and phosphates close the cycle of automated oligonucleotide synthesis and bring it to completion. For this purpose, three different Cleavage solutions from emp BIOTECH are available. The correct choice will depend on your requirements for standard, fast or mild cleavage conditions.
Standard
Usage of one volume of conc. ammonium hydroxide (= 28 %) under sealed
conditions appropriate for removal of the protecting groups of the nucleobases
Fast
Usage of one volume of AMA under sealed conditions at 65 °C for 10 min
Mild
Usage of the required volume of Deprotection, Ultramild under sealed conditions for 8 h at room temperature.
Hazard Classifications
Skin Corr. 1B
Specific target organ toxicity - single exposure, Category 3 (STOT SE 3)
Hazardous to the aquatic environment, Category 1 (Aquatic Chronic 1)
Hazard Statements
| H314 | Causes severe skin burns and eye damage. |
| H335 | May cause respiratory irritation. |
| H410 | Very toxic to aquatic life with long lasting effects. |
Precautionairy Statements
| P261 | Avoid breathing dust/fume/gas/mist/vapours/spray. |
| P271 | Use only outdoors or in a well-ventilated area. |
| P273 | Avoid release to the environment. |
| P280 | Wear protective gloves/protective clothing/eye protection/face protection. |
| P303+P361+P353 | IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water [or shower]. |
| P305+P351+P338 | IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. |
Signal Word
Danger
WGK
WGK 2
Storage Class Code
8 B - Non-flammable corrosive materials
ADR, RID (road and railroad)
UN2672 (AMMONIA SOLUTION), 8, III
IATA-DGR (transport by air)
UN2672 (AMMONIA SOLUTION), 8, III
IMDG-Code (transport by sea)
UN2672 (AMMONIA SOLUTION), 8, III
Gefahreneinstufungen
Skin Corr. 1B
Spezifische Zielorgan-Toxizität (einmalige Exposition), Kategorie 3 (STOT SE 3)
Gewässergefährdend, Kategorie 1 (Aquatic Chronic 1)
Gefahrenhinweise - H-Sätze
| H314 | Verursacht schwere Verätzungen der Haut und schwere Augenschäden. |
| H335 | Kann die Atemwege reizen. |
| H410 | Sehr giftig für Wasserorganismen, mit langfristiger Wirkung. |
Sicherheitshinweise - P-Sätze
| P261 | Einatmen von Staub/Rauch/Gas/Nebel/Dampf/Aerosol vermeiden. |
| P271 | Nur im Freien oder in gut belüfteten Räumen verwenden. |
| P273 | Freisetzung in die Umwelt vermeiden. |
| P280 | Schutzhandschuhe/Schutzkleidung/Augenschutz/Gesichtsschutz/Gehörschutz/… tragen. |
| P303+P361+P353 | BEI BERÜHRUNG MIT DER HAUT (oder dem Haar): Alle kontaminierten Kleidungsstücke sofort ausziehen. Haut mit Wasser abwaschen [oder duschen]. |
| P305+P351+P338 | BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Eventuell vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen. |
Signalwort
Gefahr
WGK
WGK 2
Lagerklasse
8 B - Nicht brennbare ätzende Stoffe
ADR, RID (Strasse und Eisenbahn)
UN2672 (AMMONIA SOLUTION), 8, III
IATA-DGR (Luftverkehr)
UN2672 (AMMONIA SOLUTION), 8, III
IMDG (Seeschiffverkehr)
UN2672 (AMMONIA SOLUTION), 8, III