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ISO 9001:2015


ID 9108613732

Fluorescein-5-isothiocyanate (5-FITC)


Cat. No.
AF-0117-D050.0-001
Content
Amount
 
 
3326-32-7
222-042-0
C21H11NO5S
389,4 g/mol
494 nm (ε =72000 M-1cm-1)
519 nm

Amine-reactive fluorescein dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 494 nm, Em: 519 nm).

This classic amine-reactive fluorescein derivatization reagent is widely used for covalent labeling of proteins and alkylamino modified nucleic acids.

Fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser, making it the predominant fluorophore for confocal laser scanning microscopy and flow cytometry applications. It has a relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility. Importantly, protein-fluorescein conjugates are not inordinately susceptible to precipitation.



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