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Management System
ISO 9001:2008


ID 9108613732

6-Carboxyfluorescein NHS ester, single isomer (6-FAM-SE)


Cat. No.
AF-0106-D005.0-001
Content
Amount
 
 
92557-81-8
C25H15NO9
473,4 g/mol
497 nm (ε = 63.000 M-1cm-1) at pH >8
519 nm at pH >8

Amine-reactive fluorescein dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 497 nm, Em: 519 nm).

This single isomer amine-reactive derivatization reagent may be used for covalent labeling of proteins and alkylamino modified nucleic acids.

Fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser, making it the predominant fluorophore for confocal laser scanning microscopy and flow cytometry applications. It has a relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility. Importantly, protein-fluorescein conjugates are not inordinately susceptible to precipitation.

 

Safety Information

Not a hazardous substance or mixture according to GHS [Regulation (EC) No. 1272/2008]. This substance or mixture is not classified as dangerous according to Directive 67/548/EEC. The product does not need to be labelled in accordance with EC directives or respective national laws.



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