Centri•Pure P96 Gel Filtration Column Arrays are designed for rapid and efficient removal of small molecules (salts, dyes, ammonia, haptens, biotin, etc.) from antibodies, enzymes and other proteins larger than 5 kD.
The gel matrix of Centri•Pure is Zetadex-25, a beaded composite material developed by emp Biotech composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability.
Molecules purified with Zetadex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal.
The size exclusion cut-off for Zetadex-25 is 5 kD for proteins. Proteins larger than 5 kD in a typical sample volume of between 150 to 300 µL can be processed with an elution volume of 300 to 500µL.
Automation of 96 Gel Column Array for Size-Exclusion & Desalting of Chlorophyll on VERSA 10 Workstation
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