| CAS-Number: | 27072-45-3 |
|---|---|
| Molecular Weight: | 389.38 |
| Empirical Formula: | C21H11NO5S |
| Absorption [nm]: | 494 ± 3 |
| Emission [nm]: | 518 ± 3 |
| Quality Control: | ≥ 95 % (by HPLC at 240 nm) |
| ε [M-1 cm-1]: | >70 000 |
| Buffer System for Optical Spectroscopy: | T&S Buffer, pH 9 |
| Solubility: | DMSO, DMF |
| Appearance (colour): | Orange |
| Appearance (form): | Solid (powder) |
| Storage: | - 20 °C. Keep dry! Keep away from light, especially in solution! |
| Trade name: | 5/6 FITC, mixed isomers |
| Reactive Moiety: | Isothiocyanate, reacts with amines to form a stable thiourea linkage |
Fluorescein-5(6)-isothiocyanate (5(6)-FITC) is a bright green dye with excitation/emission maxima of 492/517 nm. It offers a high quantum yield, is fully compatible with fluorescein (FITC) filter sets, and closely matches the 488 nm spectral line of the argon-ion laser. Common applications include labeling peptides, proteins, oligonucleotides, and other biomolecules for fluorescence microscopy, flow cytometry and in-vitro assays.
This Isothiocyanate (ITC) activated derivative enables covalent modification of primary and secondary amino groups in peptides, proteins, amino-modified nucleotides, and other amine-containing biomolecules, yielding thiourea bonds. This S-C bond is generally stable but can be destabilized by water and alkaline conditions. The product is provided as mixture of isomers.
Hazard Classifications
Respiratory/skin sensitisation, Category 1 (Resp. Sens. 1)
Hazard Statements
| H334 | May cause allergy or asthma symptoms or breathing difficulties if inhaled. |
Precautionairy Statements
| P261 | Avoid breathing dust/fume/gas/mist/vapours/spray. |
| P285 | In case of inadequate ventilation wear respiratory protection. |
| P342+P311 | If experiencing respiratory symptoms: Call a POISON CENTER/doctor/… |
Signal Word
Danger
WGK
WGK 3
Storage Class Code
11 - Flammable solids
ADR, RID (road and railroad)
Not dangerous goods
IATA-DGR (transport by air)
Not dangerous goods
IMDG-Code (transport by sea)
Not dangerous goods
Gefahreneinstufungen
Sensibilisierung der Atemwege/Haut, Kategorie 1 (Resp. Sens. 1)
Gefahrenhinweise - H-Sätze
| H334 | Kann bei Einatmen Allergie, asthmaartige Symptome oder Atembeschwerden verursachen. |
Sicherheitshinweise - P-Sätze
| P261 | Einatmen von Staub/Rauch/Gas/Nebel/Dampf/Aerosol vermeiden. |
| P285 | Bei unzureichender Belüftung Atemschutz tragen. |
| P342+P311 | Bei Symptomen der Atemwege: GIFTINFORMATIONSZENTRUM/Arzt/… anrufen. |
Signalwort
Gefahr
WGK
WGK 3
Lagerklasse
11 - Entzündbare Feststoffe
ADR, RID (Strasse und Eisenbahn)
Kein Gefahrgut
IATA-DGR (Luftverkehr)
Kein Gefahrgut
IMDG (Seeschiffverkehr)
Kein Gefahrgut