| Molecular Weight: | 722,11 |
|---|---|
| Empirical Formula: | C30H32N5O12SCl |
| Absorption [nm]: | 669 ± 3 |
| Emission [nm]: | 686 ± 3 |
| ε [M-1 cm-1]: | >53 000 |
| Buffer System for Fluorescence Readings: | TE Buffer, pH 7.6 |
| Solubility: | Acetonitrile |
| Appearance (colour): | dark blue |
| Appearance (form): | solid (powder) |
| Storage: | - 20 °C. Keep dry! Keep away from light, especially in solution! |
| Trade name: | Dicarboxymethylene blue succinimidyl ester; Dicarboxymethylene blue NHS ester |
| Reactive Moiety: | NHS ester, reacts with 1° amines to form a stable amide linkage |
The amine-reactive methylene blue derivative is used as an electroactive labeling reagent for covalent attachment to proteins and alkylamino modified nucleic acids. Dicarboxymethylene blue contains two activated carboxylic acid functional groups. In addition to its relatively high absorption, DCMB has excellent and validated redox properties and is a good generator for singlet oxygen, making it to a particularly suitable label for electrochemical and photosensitzer applications.
*Absorption and fluorescence emission maxima of dicarboxymethylene blue (DCMB, F-0201) in TE buffer pH 7.6.
Formal Electrode Potential vs. Ag/AgCl (1 M KCl): Ef = -0.216 V
Hazard Classifications
Acute Toxicity, Category 4 (Acute Tox. 4)
Skin irritation, Category 2 (Skin Irrit. 2)
Eye irritation, Category 2 (Eye Irrit. 2)
Specific target organ toxicity - single exposure, Category 3 (STOT SE 3)
Hazard Statements
| H302 | Harmful if swallowed. |
| H315 | Causes skin irritation. |
| H319 | Causes serious eye irritation. |
| H335 | May cause respiratory irritation. |
Precautionairy Statements
| P261 | Avoid breathing dust/fume/gas/mist/vapours/spray. |
| P305+P351+P338 | IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. |
Signal Word
Warning
WGK
WGK 3
Storage Class Code
10 - Flammable liquids if not LGK 3A or 3B
11 - Flammable solids
12 - Non-flammable liquids in non-flammable packages
13 - Non-flammable solids in non-flammable package
ADR, RID (road and railroad)
Not dangerous goods
IATA-DGR (transport by air)
Not dangerous goods
IMDG-Code (transport by sea)
Not dangerous goods
Gefahreneinstufungen
Akute Toxizität, Kategorie 4 (Acute Tox. 4)
Ätz-/Reizwirkung auf die Haut, Kategorie 2 (Skin Irrit. 2)
Schwere Augenschädigung/Augenreizung, Kategorie 2 (Eye Irrit. 2)
Spezifische Zielorgan-Toxizität (einmalige Exposition), Kategorie 3 (STOT SE 3)
Gefahrenhinweise - H-Sätze
| H302 | Gesundheitsschädlich bei Verschlucken. |
| H315 | Verursacht Hautreizungen. |
| H319 | Verursacht schwere Augenreizung. |
| H335 | Kann die Atemwege reizen. |
Sicherheitshinweise - P-Sätze
| P261 | Einatmen von Staub/Rauch/Gas/Nebel/Dampf/Aerosol vermeiden. |
| P305+P351+P338 | BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Eventuell vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen. |
Signalwort
Achtung
WGK
WGK 3
Lagerklasse
10 - Brennbare Flüssigkeiten soweit nicht LGK 3A bzw. LGK 3B
11 - Entzündbare Feststoffe
12 - Nicht brandgefährliche Flüssigkeiten in nicht brandgefährlicher Verpackung
13 - Nicht brandgefährliche Feststoffe in nicht brandgefährlicher Verpackung
ADR, RID (Strasse und Eisenbahn)
Kein Gefahrgut
IATA-DGR (Luftverkehr)
Kein Gefahrgut
IMDG (Seeschiffverkehr)
Kein Gefahrgut