CAS-Number: | 27072-45-3 |
---|---|
Molecular Weight: | 389.38 |
Empirical Formula: | C21H11NO5S |
Absorption [nm]: | 494 ± 3 |
Emission [nm]: | 518 ± 3 |
ε [M-1 cm-1]: | >70 000 |
Buffer System for Fluorescence Readings: | T&S Buffer, pH 9 |
Solubility: | DMF |
Appearance (colour): | orange |
Appearance (form): | solid (powder) |
Storage: | - 20 °C. Keep dry! Keep away from light, especially in solution! |
Trade name: | 5/6 FITC, mixed isomers |
Reactive Moiety: | Isothiocyanate, reacts with 1° amines to form a stable thiourea linkage |
This classic amine-reactive fluorescein derivatization reagent is widely used for covalent labeling of proteins and alkylamino modified nucleic acids.
Fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser, making it the predominant fluorophore for confocal laser scanning microscopy and flow cytometry applications. It has a relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility. Importantly, protein-fluorescein conjugates are not inordinately susceptible to precipitation.
5/6-FITC
Respiratory/skin sensitisation, Category 1 (Resp. Sens. 1)
H334 | May cause allergy or asthma symptoms or breathing difficulties if inhaled. |
P342+P311 | If experiencing respiratory symptoms: Call a POISON CENTER/doctor/… |
P261 | Avoid breathing dust/fume/gas/mist/vapours/spray. |
P285 | In case of inadequate ventilation wear respiratory protection. |
Danger
WGK 3
11 - Flammable solids
Not dangerous goods
Not dangerous goods
Not dangerous goods
Sensibilisierung der Atemwege/Haut, Kategorie 1 (Resp. Sens. 1)
H334 | Kann bei Einatmen Allergie, asthmaartige Symptome oder Atembeschwerden verursachen. |
P342+P311 | Bei Symptomen der Atemwege: GIFTINFORMATIONSZENTRUM/Arzt/… anrufen. |
P261 | Einatmen von Staub/Rauch/Gas/Nebel/Dampf/Aerosol vermeiden. |
P285 | Bei unzureichender Belüftung Atemschutz tragen. |
Gefahr
WGK 3
11 - Entzündbare Feststoffe
Kein Gefahrgut
Kein Gefahrgut
Kein Gefahrgut