| CAS-Number: | 76823-03-5 |
|---|---|
| Molecular Weight: | 376,32 |
| Empirical Formula: | C21H12O7 |
| Absorption [nm]: | 493 ± 3 |
| Emission [nm]: | 517 ± 3 |
| ε [M-1 cm-1]: | >72 000 |
| Excitation [nm]: | 488 |
| Buffer System for Fluorescence Readings: | PBS, pH 7.4 |
| Solubility: | Methanol, DMF, DMSO |
| Appearance (colour): | yellow |
| Appearance (form): | solid (powder) |
| Storage: | RT! Keep dry! Keep away from light, especially in solution! |
| Trade name: | 5-Carboxyfluorescein; 5-FAM; 5-Carboxyfluorescein, single isomer |
| Reactive Moiety: | free carboxylic acid |
This single isomer non-activated fluorescein derivatization reagent may be used for covalent labeling of proteins and alkylamino modified nucleic acids.
Fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser, making it the predominant fluorophore for confocal laser scanning microscopy and flow cytometry applications. It has a relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility. Importantly, protein-fluorescein conjugates are not inordinately susceptible to precipitation.
5-FAM
Absorption: 493 nm (e = 72.000 M-1cm-1) at pH >8
Emission: 517 nm at pH >8
Hazard Classifications
Eye irritation, Category 2 (Eye Irrit. 2)
Hazard Statements
| H319 | Causes serious eye irritation. |
Precautionairy Statements
| P262 | Do not get in eyes, on skin, or on clothing. |
| P280 | Wear protective gloves/protective clothing/eye protection/face protection. |
| P305+P351+P338 | IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. |
Signal Word
Warning
WGK
WGK 3
Storage Class Code
13 - Non-flammable solids in non-flammable package
ADR, RID (road and railroad)
Not dangerous goods
IATA-DGR (transport by air)
Not dangerous goods
IMDG-Code (transport by sea)
Not dangerous goods
Gefahreneinstufungen
Schwere Augenschädigung/Augenreizung, Kategorie 2 (Eye Irrit. 2)
Gefahrenhinweise - H-Sätze
| H319 | Verursacht schwere Augenreizung. |
Sicherheitshinweise - P-Sätze
| P262 | Nicht in die Augen, auf die Haut oder auf die Kleidung gelangen lassen. |
| P280 | Schutzhandschuhe/Schutzkleidung/Augenschutz/Gesichtsschutz/Gehörschutz/… tragen. |
| P305+P351+P338 | BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Eventuell vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen. |
Signalwort
Achtung
WGK
WGK 3
Lagerklasse
13 - Nicht brandgefährliche Feststoffe in nicht brandgefährlicher Verpackung
ADR, RID (Strasse und Eisenbahn)
Kein Gefahrgut
IATA-DGR (Luftverkehr)
Kein Gefahrgut
IMDG (Seeschiffverkehr)
Kein Gefahrgut