| CAS-Number: | 28718-90-3 |
|---|---|
| Molecular Weight: | 350,25 |
| Empirical Formula: | C16H17N5Cl2 |
| Absorption [nm]: | 343 ± 3 |
| Emission [nm]: | 444 ± 3 |
| ε [M-1 cm-1]: | >30 000 |
| Buffer System for Fluorescence Readings: | H2O |
| Solubility: | H2O |
| Appearance (colour): | yellow |
| Appearance (form): | solution |
| Storage: | +2-8 °C. Keep away from light! |
| Trade name: | 4',6-Diamidino-2-phenyl-indol dihydrochloride; DAPI, Dihydrochloride |
DAPI aqueous solution from emp Biotech is a high-purity reagent for fixed-cell, fluorescent staining of DNA content and cell nuclei, formulated specifically for cellular imaging techniques.
A classic nuclear counterstain used in immunofluorescence microscopy, DAPI (diamidino-2-phenylindole) is an important component of high-content screening methods which require cell-based quantization of DNA content.
DAPI exhibits blue fluorescence that strongly increases after it selectively binds to the minor groove of double stranded DNA. The fluorescence of bound DAPI is approximately 20-fold greater than in its unbound state. This selectivity, combined with DAPI#s high cell permeability, allows effective staining of nuclei and low fluorescence background from the cytoplasm.
DAPI is compatible with antibodies and other probes which have been labeled with fluorescein and rhodamine, and can be used in tandem as a counterstain with such probes. The used of DAPI is limited to fixed cells. For live cell imaging, we recommend Hoechst 33342.
CAS: 28718-90-3
Excitation: 341 nm (360 nm with dsDNA)
Emission: 452 nm (458 nm with dsDNA)
Hazard Classifications
Skin irritation, Category 2 (Skin Irrit. 2)
Respiratory/skin sensitisation, Category 1A (Skin Irrit. 1A)
Specific target organ toxicity - single exposure, Category 3 (STOT SE 3)
Hazard Statements
| H315 | Causes skin irritation. |
| H317 | May cause an allergic skin reaction. |
| H335 | May cause respiratory irritation. |
Precautionairy Statements
| P261 | Avoid breathing dust/fume/gas/mist/vapours/spray. |
| P262 | Do not get in eyes, on skin, or on clothing. |
| P272 | Contaminated work clothing should not be allowed out of the workplace. |
| P280 | Wear protective gloves/protective clothing/eye protection/face protection. |
| P302+P352 | IF ON SKIN: Wash with plenty of water/… |
| P333+P313 | If skin irritation or rash occurs: Get medical advice/attention. |
| P362+P364 | Take off contaminated clothing and wash it before reuse. |
Signal Word
Warning
WGK
WGK 1
Storage Class Code
12 - Non-flammable liquids in non-flammable packages
ADR, RID (road and railroad)
Not dangerous goods
IATA-DGR (transport by air)
Not dangerous goods
IMDG-Code (transport by sea)
Not dangerous goods
Gefahreneinstufungen
Ätz-/Reizwirkung auf die Haut, Kategorie 2 (Skin Irrit. 2)
Sensibilisierung der Atemwege/Haut, Kategorie 1A (Skin Irrit. 1A)
Spezifische Zielorgan-Toxizität (einmalige Exposition), Kategorie 3 (STOT SE 3)
Gefahrenhinweise - H-Sätze
| H315 | Verursacht Hautreizungen. |
| H317 | Kann allergische Hautreaktionen verursachen. |
| H335 | Kann die Atemwege reizen. |
Sicherheitshinweise - P-Sätze
| P261 | Einatmen von Staub/Rauch/Gas/Nebel/Dampf/Aerosol vermeiden. |
| P262 | Nicht in die Augen, auf die Haut oder auf die Kleidung gelangen lassen. |
| P272 | Kontaminierte Arbeitskleidung nicht außerhalb des Arbeitsplatzes tragen. |
| P280 | Schutzhandschuhe/Schutzkleidung/Augenschutz/Gesichtsschutz/Gehörschutz/… tragen. |
| P302+P352 | BEI BERÜHRUNG MIT DER HAUT: Mit viel Wasser/… waschen. |
| P333+P313 | IBei Hautreizung oder -ausschlag: Ärztlichen Rat einholen/ärztliche Hilfe hinzuziehen. |
| P362+P364 | Kontaminierte Kleidung ausziehen und vor erneutem Tragen waschen. |
Signalwort
Achtung
WGK
WGK 1
Lagerklasse
12 - Nicht brandgefährliche Flüssigkeiten in nicht brandgefährlicher Verpackung
ADR, RID (Strasse und Eisenbahn)
Kein Gefahrgut
IATA-DGR (Luftverkehr)
Kein Gefahrgut
IMDG (Seeschiffverkehr)
Kein Gefahrgut