Molecular Weight: | 439.5 |
---|---|
Empirical Formula: | C22H23N4O8SCl |
Absorption [nm]: | 665 ± 3 |
Emission [nm]: | 670 ± 3 |
ε [M-1 cm-1]: | 81 000 |
Storage: | Store at -20 °C. Keep dry. Protect from light! |
Reactive Moiety: | NHS ester, reacts with 1° amines to form a stable amide linkage |
TurboTag 667 Methylene Blue Protein Labeling & Purification Kit is designed for the labeling of proteins with molecular weights greater than 25 kD (in particular antibodies), using a reactive succinimidyl-ester of dicarboxy Methylene Blue. The conjugates result from the formation of a stable covalent amide linkage.
The protein-dye conjugates have fluorescence-excitation at around 651 nm and a very weak fluorescence-emission maxima at 666 nm. Due to its weak fluorescence, Methylene Blue is used primarily as a Redox Dye, an intercalator of nucleic acids or as an electroactive marker rather than as a fluorescence marker. Up to 15 nmol of protein can be labeled using one vial (50 nmol) of the reactive TurboTag 667 methylene blue dye.
To get a quick start with IgG, use the Optimized Protocol for Labeling 1 mg of IgG or the Optimized Protocol for Labeling 100 µg of IgG (i.e. monoclonal antibodies).
Hazard Classifications
Serious eye damage/eye irritation, Category 1 (Eye Dam. 1)
Hazard Statements
H318 | Causes serious eye damage. |
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Precautionairy Statements
P280 | Wear protective gloves/protective clothing/eye protection/face protection. |
P305+P351+P338 | IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. |
P310 | Immediately call a POISON CENTER/doctor/… |
Signal Word
Danger
WGK
-
Storage Class Code
10 - Flammable liquids if not LGK 3A or 3B
11 - Flammable solids
12 - Non-flammable liquids in non-flammable packages
13 - Non-flammable solids in non-flammable package
ADR, RID (road and railroad)
Not dangerous goods
IATA-DGR (transport by air)
Not dangerous goods
IMDG-Code (transport by sea)
Not dangerous goods
Gefahreneinstufungen
Schwere Augenschädigung/Augenreizung, Kategorie 1 (Eye Dam. 1)
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Gefahrenhinweise - H-Sätze
H318 | Verursacht schwere Augenschäden |
Sicherheitshinweise - P-Sätze
P280 | Schutzhandschuhe/Schutzkleidung/Augenschutz/Gesichtsschutz/Gehörschutz/… tragen. |
P305+P351+P338 | BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Eventuell vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen. |
P310 | Sofort GIFTINFORMATIONSZENTRUM/Arzt/… anrufen. |
Signalwort
Gefahr
WGK
-
Lagerklasse
10 - Brennbare Flüssigkeiten soweit nicht LGK 3A bzw. LGK 3B
11 - Entzündbare Feststoffe
12 - Nicht brandgefährliche Flüssigkeiten in nicht brandgefährlicher Verpackung
13 - Nicht brandgefährliche Feststoffe in nicht brandgefährlicher Verpackung
ADR, RID (Strasse und Eisenbahn)
Kein Gefahrgut
IATA-DGR (Luftverkehr)
Kein Gefahrgut
IMDG (Seeschiffverkehr)
Kein Gefahrgut