CentriPure N96+ Gel Filtration Column Arrays are designed for rapid and efficient removal of small molecules (salts, dyes, ammonia, haptens, biotin, etc.) from nucleic acids and oligonucleotides longer than 10 base pairs.
The size exclusion cut-off for Zetadex-25 is 10 base pairs for oligonucleotides. Nucleic acids larger than 10 bp in a typical sample volume of 500 µL can be processed with an elution volume of approximately 800 µL.
The gel matrix of CentriPure is Zetadex-25, a beaded composite material developed by emp Biotech composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability.
Molecules purified with Zetadex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal.This item is renamed to CentriPure 96 Gel Filtration Column Array 500 - Z25M