| Molecular Weight: | 538,96 |
|---|---|
| Empirical Formula: | C22H23N4O8SCl |
| Absorption [nm]: | 665 ± 3 |
| Emission [nm]: | 670 ± 3 |
| ε [M-1 cm-1]: | >81 000 |
| Solubility: | Acetonitrile |
| Appearance (colour): | dark blue |
| Appearance (form): | solid (powder) |
| Storage: | - 20 °C. Keep dry! Keep away from light, especially in solution! |
| Trade name: | Monocarboxymethylene blue succinimidyl ester; Monocarboxymethylene blue NHS ester; Methylene blue NHS ester; Methylene blue succinimidyl ester |
| Reactive Moiety: | NHS ester, reacts with 1° amines to form a stable amide linkage |
The amine-reactive methylene blue derivative is used as an electroactive labeling reagent for covalent attachment to proteins and alkylamino modified nucleic acids. Monocarboxymethylene blue contains a single activated carboxylic acid functional group. In addition to its relatively high absorption, MCMB, has excellent and validated redox properties and is a good generator for singlet oxygen, making it to a particularly suitable label for electrochemical and photosensitzer applications.
MCMB-SE
CAS: none known
*Absorption and fluorescence emission maxima of monocarboxymethylene blue (MCMB, F-0202) in TE buffer pH 7.6
Formal Electrode Potential vs. Ag/AgCl (1 M KCl): Ef = -0.198 V
Hazard Classifications
Acute Toxicity, Category 4 (Acute Tox. 4)
Skin irritation, Category 2 (Skin Irrit. 2)
Eye irritation, Category 2 (Eye Irrit. 2)
Specific target organ toxicity - single exposure, Category 3 (STOT SE 3)
Hazard Statements
| H302 | Harmful if swallowed. |
| H315 | Causes skin irritation. |
| H319 | Causes serious eye irritation. |
| H335 | May cause respiratory irritation. |
Precautionairy Statements
| P261 | Avoid breathing dust/fume/gas/mist/vapours/spray. |
| P305+P351+P338 | IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. |
Signal Word
Warning
WGK
WGK 3
Storage Class Code
10 - Flammable liquids if not LGK 3A or 3B
11 - Flammable solids
12 - Non-flammable liquids in non-flammable packages
13 - Non-flammable solids in non-flammable package
ADR, RID (road and railroad)
Not dangerous goods
IATA-DGR (transport by air)
Not dangerous goods
IMDG-Code (transport by sea)
Not dangerous goods
Gefahreneinstufungen
Akute Toxizität, Kategorie 4 (Acute Tox. 4)
Ätz-/Reizwirkung auf die Haut, Kategorie 2 (Skin Irrit. 2)
Schwere Augenschädigung/Augenreizung, Kategorie 2 (Eye Irrit. 2)
Spezifische Zielorgan-Toxizität (einmalige Exposition), Kategorie 3 (STOT SE 3)
Gefahrenhinweise - H-Sätze
| H302 | Gesundheitsschädlich bei Verschlucken. |
| H315 | Verursacht Hautreizungen. |
| H319 | Verursacht schwere Augenreizung. |
| H335 | Kann die Atemwege reizen. |
Sicherheitshinweise - P-Sätze
| P261 | Einatmen von Staub/Rauch/Gas/Nebel/Dampf/Aerosol vermeiden. |
| P305+P351+P338 | BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Eventuell vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen. |
Signalwort
Achtung
WGK
WGK 3
Lagerklasse
10 - Brennbare Flüssigkeiten soweit nicht LGK 3A bzw. LGK 3B
11 - Entzündbare Feststoffe
12 - Nicht brandgefährliche Flüssigkeiten in nicht brandgefährlicher Verpackung
13 - Nicht brandgefährliche Feststoffe in nicht brandgefährlicher Verpackung
ADR, RID (Strasse und Eisenbahn)
Kein Gefahrgut
IATA-DGR (Luftverkehr)
Kein Gefahrgut
IMDG (Seeschiffverkehr)
Kein Gefahrgut