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Cleavage of the oligonucleotide from its solid support and subsequent removal of all protecting groups from the nucleobases and phosphates close the cycle of automated oligonucleotide synthesis and bring it to completion. For this purpose, three different Cleavage solutions from emp BIOTECH are available. The correct choice will depend on your requirements for standard, fast or mild cleavage conditions.
Standard: Usage of one volume of conc. ammonium hydroxide (≥ 28 %) under sealed conditions appropriate for removal of the protecting groups of the nucleobases Fast: Usage of one volume of AMA under sealed conditions at 65 °C for 10 min Mild: Usage of the required volume of Deprotection, Ultramild under sealed conditions for 8 h at room temperature
Hazard Classifications
Flammable liquid, Category 2 (Flam. Liq. 2)
Skin irritation, Category 2 (Skin Irrit. 2)
Serious eye damage/eye irritation, Category 1 (Eye Dam. 1)
Hazardous to the aquatic environment, Category 1 (Aquatic Chronic 1)
Hazard Statements
| H225 | Highly flammable liquid and vapour. |
| H315 | Causes skin irritation. |
| H318 | Causes serious eye damage. |
| H410 | Very toxic to aquatic life with long lasting effects. |
Precautionairy Statements
| P210 | Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. |
| P233 | Keep container tightly closed. |
| P273 | Avoid release to the environment. |
| P280 | Wear protective gloves/protective clothing/eye protection/face protection. |
| P303+P361+P353 | IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water [or shower]. |
| P305+P351+P338 | IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. |
Signal Word
Danger
WGK
WGK 1
Storage Class Code
3 - Flammable liquids
ADR, RID (road and railroad)
UN2924 FLAMMABLE LIQUID, CORROSIVE, N.O.S. (Mixture of Ethanol and conc. Ammonia), 3(8), II
IATA-DGR (transport by air)
UN2924 FLAMMABLE LIQUID, CORROSIVE, N.O.S. (Mixture of Ethanol and conc. Ammonia), 3(8), II
IMDG-Code (transport by sea)
UN2924 FLAMMABLE LIQUID, CORROSIVE, N.O.S. (Mixture of Ethanol and conc. Ammonia), 3(8), II
Gefahreneinstufungen
Entzündbare Flüssigkeiten, Kategorie 2 (Flam. Liq. 2)
Ätz-/Reizwirkung auf die Haut, Kategorie 2 (Skin Irrit. 2)
Schwere Augenschädigung/Augenreizung, Kategorie 1 (Eye Dam. 1)
Gewässergefährdend, Kategorie 1 (Aquatic Chronic 1)
Gefahrenhinweise - H-Sätze
| H225 | Flüssigkeit und Dampf leicht entzündbar. |
| H315 | Verursacht Hautreizungen. |
| H318 | Verursacht schwere Augenschäden |
| H410 | Sehr giftig für Wasserorganismen, mit langfristiger Wirkung. |
Sicherheitshinweise - P-Sätze
| P210 | Von Hitze, heißen Oberflächen, Funken, offenen Flammen und anderen Zündquellen fernhalten. Nicht rauchen. |
| P233 | Behälter dicht verschlossen halten. |
| P273 | Freisetzung in die Umwelt vermeiden. |
| P280 | Schutzhandschuhe/Schutzkleidung/Augenschutz/Gesichtsschutz/Gehörschutz/… tragen. |
| P303+P361+P353 | BEI BERÜHRUNG MIT DER HAUT (oder dem Haar): Alle kontaminierten Kleidungsstücke sofort ausziehen. Haut mit Wasser abwaschen [oder duschen]. |
| P305+P351+P338 | BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Eventuell vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen. |
Signalwort
Gefahr
WGK
WGK 1
Lagerklasse
3 - Entzündliche flüssige Stoffe
ADR, RID (Strasse und Eisenbahn)
UN2924 FLAMMABLE LIQUID, CORROSIVE, N.O.S. (Mixture of Ethanol and conc. Ammonia), 3(8), II
IATA-DGR (Luftverkehr)
UN2924 FLAMMABLE LIQUID, CORROSIVE, N.O.S. (Mixture of Ethanol and conc. Ammonia), 3(8), II
IMDG (Seeschiffverkehr)
UN2924 FLAMMABLE LIQUID, CORROSIVE, N.O.S. (Mixture of Ethanol and conc. Ammonia), 3(8), II