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Cleavage of the oligonucleotide from its solid support and subsequent removal of all protecting groups from the nucleobases and phosphates close the cycle of automated oligonucleotide synthesis and bring it to completion. For this purpose, three different Cleavage solutions from emp BIOTECH are available. The correct choice will depend on your requirements for standard, fast or mild cleavage conditions.
Standard: Usage of one volume of conc. ammonium hydroxide (≥ 28 %) under sealed conditions appropriate for removal of the protecting groups of the nucleobases Fast: Usage of one volume of AMA under sealed conditions at 65 °C for 10 min Mild: Usage of the required volume of Deprotection, Ultramild under sealed conditions for 8 h at room temperature
Hazard Classifications
Flammable liquid, Category 2 (Flam. Liq. 2)
Substance or mixture corrosive to metals, Category 1 (Met. Corr. 1)
Acute Toxicity, Category 4 (Acute Tox. 4)
Skin Corr. 1
Serious eye damage/eye irritation, Category 1 (Eye Dam. 1)
Acute Toxicity, Category 3 (Acute Tox. 3)
Specific target organ toxicity - single exposure, Category 3 (STOT SE 3)
Hazardous to the aquatic environment, Category 1 (Aquatic Acute 1)
Hazard Statements
| H225 | Highly flammable liquid and vapour. |
| H290 | May be corrosive to metals. |
| H302 | Harmful if swallowed. |
| H314 | Causes severe skin burns and eye damage. |
| H318 | Causes serious eye damage. |
| H331 | Toxic if inhaled. |
| H335 | May cause respiratory irritation. |
| H400 | Very toxic to aquatic life. |
Precautionairy Statements
| P210 | Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. |
| P260 | Do not breathe dust/fume/gas/mist/vapours/spray. |
| P273 | Avoid release to the environment. |
| P280 | Wear protective gloves/protective clothing/eye protection/face protection. |
| P303+P361+P353 | IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water [or shower]. |
| P304+P340 | IF INHALED: Remove person to fresh air and keep comfortable for breathing. |
| P305+P351+P338 | IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. |
| P403+P233 | Store in a well-ventilated place. Keep container tightly closed. |
Signal Word
Danger
WGK
WGK 2
Storage Class Code
8 A - Flammable corrosive materials
ADR, RID (road and railroad)
UN2924 FLAMMABLE LIQUID, CORROSIVE, N.O.S. (Aqueous solution of ammonia and methylamine), 3(8), II
IATA-DGR (transport by air)
UN2924 FLAMMABLE LIQUID, CORROSIVE, N.O.S. (Aqueous solution of ammonia and methylamine), 3(8), II
IMDG-Code (transport by sea)
UN2924 FLAMMABLE LIQUID, CORROSIVE, N.O.S. (Aqueous solution of ammonia and methylamine), 3(8), II
Gefahreneinstufungen
Entzündbare Flüssigkeiten, Kategorie 2 (Flam. Liq. 2)
Auf Metalle korrosiv wirkende Stoffe oder Gemische, Kategorie 1 (Met. Corr. 1)
Akute Toxizität, Kategorie 4 (Acute Tox. 4)
Skin Corr. 1
Schwere Augenschädigung/Augenreizung, Kategorie 1 (Eye Dam. 1)
Akute Toxizität, Kategorie 3 (Acute Tox. 3)
Spezifische Zielorgan-Toxizität (einmalige Exposition), Kategorie 3 (STOT SE 3)
Gewässergefährdend, Kategorie 1 (Aquatic Acute 1)
Gefahrenhinweise - H-Sätze
| H225 | Flüssigkeit und Dampf leicht entzündbar. |
| H290 | Kann gegenüber Metallen korrosiv sein. |
| H302 | Gesundheitsschädlich bei Verschlucken. |
| H314 | Verursacht schwere Verätzungen der Haut und schwere Augenschäden. |
| H318 | Verursacht schwere Augenschäden |
| H331 | Giftig bei Einatmen. |
| H335 | Kann die Atemwege reizen. |
| H400 | Sehr giftig für Wasserorganismen. |
Sicherheitshinweise - P-Sätze
| P210 | Von Hitze, heißen Oberflächen, Funken, offenen Flammen und anderen Zündquellen fernhalten. Nicht rauchen. |
| P260 | Staub/Rauch/Gas/Nebel/Dampf/Aerosol nicht einatmen. |
| P273 | Freisetzung in die Umwelt vermeiden. |
| P280 | Schutzhandschuhe/Schutzkleidung/Augenschutz/Gesichtsschutz/Gehörschutz/… tragen. |
| P303+P361+P353 | BEI BERÜHRUNG MIT DER HAUT (oder dem Haar): Alle kontaminierten Kleidungsstücke sofort ausziehen. Haut mit Wasser abwaschen [oder duschen]. |
| P304+P340 | BEI EINATMEN: Die Person an die frische Luft bringen und für ungehinderte Atmung sorgen. |
| P305+P351+P338 | BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Eventuell vorhandene Kontaktlinsen nach Möglichkeit entfernen. Weiter spülen. |
| P403+P233 | An einem gut belüfteten Ort aufbewahren. Behälter dicht verschlossen halten. |
Signalwort
Gefahr
WGK
WGK 2
Lagerklasse
8 A - Brennbare ätzende Stoffe
ADR, RID (Strasse und Eisenbahn)
UN2924 FLAMMABLE LIQUID, CORROSIVE, N.O.S. (Aqueous solution of ammonia and methylamine), 3(8), II
IATA-DGR (Luftverkehr)
UN2924 FLAMMABLE LIQUID, CORROSIVE, N.O.S. (Aqueous solution of ammonia and methylamine), 3(8), II
IMDG (Seeschiffverkehr)
UN2924 FLAMMABLE LIQUID, CORROSIVE, N.O.S. (Aqueous solution of ammonia and methylamine), 3(8), II